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Effects of transcription factor silencing on transformed phenotypes expressed in KRAS-transformed cells support hierarchical regulation in the gene-regulatory network. (A) Anchorage-independent proliferation ( (3D) growth in soft agar cultures). (B) Anchorage-dependent proliferation ( (2D) growth in adherent monolayer cultures) of RAS-ROSE cells 48 after treatment with scrambled siRNA-duplex (Sc),ray ban uk, transfection reagents only (Mock) and two independent siRNA duplexes targeting each transcription factor as determined by colorimetric XTT assay.
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